Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Rosetta cells | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method. Inactive D198N mutant bound to 5'-methylthioadenosine using 0.2 M magnesium chloride, 0.1 M HEPES (pH 7.5), and 25% (w/v) PEG 3350 | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
D198N | inactive | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-amino-6-deoxyfutalosine + H2O | Helicobacter pylori | - |
dehypoxanthine futalosine + adenine | - |
? | |
additional information | Helicobacter pylori | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase is a multifunctional enzyme that catalyzes the hydrolysis of the N-ribosidic bond of at least four different adenosine-based metabolites: S-adenosyl-L-homocysteine, 5'-methylthioadenosine, 5'-deoxyadenosine, and 6-amino-6-deoxyfutalosine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-amino-6-deoxyfutalosine + H2O | - |
Helicobacter pylori | dehypoxanthine futalosine + adenine | - |
? | |
additional information | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase is a multifunctional enzyme that catalyzes the hydrolysis of the N-ribosidic bond of at least four different adenosine-based metabolites: S-adenosyl-L-homocysteine, 5'-methylthioadenosine, 5'-deoxyadenosine, and 6-amino-6-deoxyfutalosine | Helicobacter pylori | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Helicobacter pylori |
MTAN | - |
Helicobacter pylori |